{"id":10751,"date":"2023-02-10T12:35:21","date_gmt":"2023-02-10T17:35:21","guid":{"rendered":"https:\/\/qprn.ca\/2023\/02\/le-traitement-de-la-douleur-au-niveau-cellulaire-zoom-sur-la-recherche\/"},"modified":"2024-06-18T09:48:31","modified_gmt":"2024-06-18T13:48:31","slug":"le-traitement-de-la-douleur-au-niveau-cellulaire-zoom-sur-la-recherche","status":"publish","type":"post","link":"https:\/\/qprn.ca\/en\/2023\/02\/10\/le-traitement-de-la-douleur-au-niveau-cellulaire-zoom-sur-la-recherche\/","title":{"rendered":"Pain treatment at the cellular level, let\u2019s take a look through the lens!"},"content":{"rendered":"\n<h2 class=\"wp-block-heading\">Goal<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">Ortho-Vanillin (o-Vanillin) is a natural compound that we can find in essential oils of different plants. It has senolytic activity, meaning that it could remove these senescent cells, as well as anti-inflammatory properties and could be a valuable supplement to MSC and EV therapy for discogenic LBP. This study explored EV release and transfer between MSCs and intervertebral disc (IVD) cells and examined the effect on MSC differentiation and IVD cell phenotype in the presence and absence of o-Vanillin.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Methodology<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/qprn.ca\/en\/investigator\/lisbet-haglund\/\" target=\"_blank\" rel=\"noreferrer noopener\">Dr Haglund<\/a>\u2019s research team used direct co-culture models in this study. These types of models allow to grow cells in the laboratory and study how they communicate and interact between each other by releasing cellular messengers meant for certain types of cells. This allowed researchers to evaluate regeneration, inflammatory mediators, and senescent cells in cell pellets in the presence or absence of o-Vanillin. EV release (the action of releasing particles outside of the cell) and transfer between different types of cells (MSCs and IVD cells) were examined. The effects on MSC differentiation and IVD cell phenotype were also evaluated. In other words, researchers were interested to see the impact of o-Vanillin on young cells that needed to specialize and acquire mature cells characteristics to be able to perform the action they were meant to perform.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Main findings<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">This study demonstrates that o-Vanillin improved MSC differentiation towards an IVD-like cell, meaning that the MSC were able to develop into cartilage and connective tissue and perform the function they were meant to, such as protecting the space between the vertebrae. O-Vanillin also improved IVD cell phenotype. Co-cultures of MSCs and IVD cells resulted in increased matrix synthesis, fewer senescent cells and a decreased release of the cytokines IL6 and IL8. In other words, it improved the cellular environment and helped develop cells. The effects were further enhanced by o-Vanillin. o-Vanillin profoundly increased EV release and\/or uptake by MSCs and IVD cells. IVD cell markers were significantly upregulated in both cell types in response to conditioned media of o-Vanillin-treated donor cells.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Take home message<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">In order for cells to develop and accomplish the role they are supposed to, in this case protect the space between our vertebras, we need to have a certain environment in place that will help them achieve their goal. This study demonstrates that a compound like o-Vanillin affects MSC and IVD cell communication and suggests that combining MSCs and senolytic compounds may be involved in the outcome of cell supplementation and EV therapy for LBP. When the environment is thus altered, there can be a development of a disease such as LBP and researchers work hard to prevent or treat the disease.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/www.mdpi.com\/2073-4409\/11\/22\/3589\" target=\"_blank\" rel=\"noreferrer noopener\"><strong>Read more<\/strong><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Goal Ortho-Vanillin (o-Vanillin) is a natural compound that we can find in essential oils of&#8230;<\/p>\n","protected":false},"author":3,"featured_media":10748,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[69],"tags":[38],"class_list":["post-10751","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-popularized-publications","tag-articles-en"],"acf":[],"views":280,"_links":{"self":[{"href":"https:\/\/qprn.ca\/en\/wp-json\/wp\/v2\/posts\/10751","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/qprn.ca\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/qprn.ca\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/qprn.ca\/en\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/qprn.ca\/en\/wp-json\/wp\/v2\/comments?post=10751"}],"version-history":[{"count":0,"href":"https:\/\/qprn.ca\/en\/wp-json\/wp\/v2\/posts\/10751\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/qprn.ca\/en\/wp-json\/wp\/v2\/media\/10748"}],"wp:attachment":[{"href":"https:\/\/qprn.ca\/en\/wp-json\/wp\/v2\/media?parent=10751"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/qprn.ca\/en\/wp-json\/wp\/v2\/categories?post=10751"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/qprn.ca\/en\/wp-json\/wp\/v2\/tags?post=10751"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}